Bifidobacterium lactis BB-12 Attenuates Macrophage Aging Induced by D-Galactose and Promotes M2 Macrophage Polarization

Figure 5

Effects of BB-12 on J774A.1 macrophage senescence. (a) SA-β-gal staining. Compared with the D-gal group, the SA-β-gal-positive cells in the BB-12 group significantly decreased. (b) SA-β-gal-positive cell quantification. The counts showed that the number of SA-β-gal-positive cells in the D-gal+60 min BB-12 and D-gal+6 h BB-12 groups decreased significantly compared with that in the D-gal group (, ). (c) Western blot analysis of the p53 and p16 expression levels. The p53 and p16 expression levels were gradually lower in the D-gal+30 min BB-12, D-gal+60 min BB-12, and D-gal+6 h BB-12 groups compared with those in the D-gal group. β-Actin was used as the internal control.

(a)

(b)

(c)