Bifidobacterium lactis BB-12 Attenuates Macrophage Aging Induced by D-Galactose and Promotes M2 Macrophage Polarization

Figure 6

Effects of BB-12 on J774A.1 macrophage polarization. The production of IL-6 (a), IL-12 (b), and IL-10 (c) was assayed by ELISA. The results showed that the IL-6 and IL-12 levels in the D-gal+60 min and D-gal+6 h groups decreased compared with that in the D-gal group ( or , ). IL-10 in the D-gal+60 min and D-gal+6 h groups was significantly higher than that in the D-gal group (, ). (d) qPCR analysis of the relative expression level of Arg-1 mRNA. GAPDH was used as an internal control. The Arg-1 mRNA expression level in D-gal+60 min and D-gal+6 h groups increased compared with that in the D-gal group (, ).

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