The RNA-Binding Protein KSRP Modulates Cytokine Expression of CD4⁺ T Cells

Figure 5

Effect of inactivation of the KSRP gene on IFN-γ production in T cells. Magnetic bead separation was used to isolate untouched CD8⁺ T cells from KSRP^+/+ (WT) and KSRP^-/- (KO) mice. T cells were polyclonally stimulated with plate-bound anti-CD3- (1 μg/ml) and anti-CD28- (2 μg/ml) specific antibodies for 96 h. In supernatants of these cells, IFN-γ contents were measured by CBA (a). Shown data are the ( animals per genotype) and are pooled from three independent experiments (; unpaired -test). KSRP^+/+ (WT) and KSRP^-/- (KO) were treated on day 0 with a cocktail of 5 collagen-II-specific mAbs (AB) or with PBS (Ctrl) as control. On day 3, the animals were treated with LPS (50 μg/animal) or PBS as control. At day 5 and day 9, mice () of each treatment group were killed for subsequent analyses. Nylon wool-enriched T cells isolated from the spleens of KSRP^+/+ (WT) and KSRP^-/- (KO) mice treated with (AB) or without (Ctrl) CII-specific mAbs were polyclonally stimulated with anti-CD3- (1 μg/ml) and anti-CD28- (2 μg/ml) specific antibodies for 96 h. IFN-γ production of these cells was monitored by CBA (b). Data shown are the ( animals per genotype at days 5 and 9) and are pooled from four independent experiments. (; unpaired -test).

(a)

(b)