MHC Class I Molecules Exacerbate Viral Infection by Disrupting Type I Interferon Signaling

Figure 3

MHC I suppressed IFN signaling through SHP2. (a) Western blot analysis of JAK1 and SHP2 phosphorylation in WT and MHC I^-/- macrophages at the indicated times post VSV infection. (b) Relative density of the blot in (a) from 3 independent experiments. (c) VSV RNA replication (left) and TCID₅₀ (right) in WT and MHC I^-/- macrophages with Shp2 silenced before infection. (d) Western blot analysis of STAT1 activation in WT and MHC I^-/- macrophages with Shp2 silenced before infection. (e) Relative density of the blot in (d). (f) Immunoblot assay of SHP2 and STAT1 post coimmunoprecipitation with anti-STAT1. (g) Relative density of the blot in (f). Data are the of at least three independent experiments. Two-way ANOVA was adopted for statistical analysis in (b), (c), and (e). One-way ANOVA was adopted for statistical analysis in (g). .

(a)

(b)

(c)

(d)

(e)

(f)

(g)