MHC Class I Molecules Exacerbate Viral Infection by Disrupting Type I Interferon Signaling

Figure 4

MHC I promotes viral replication during the innate phase of infection in vivo. (a) Macrophages were cross-linked with anti-MHC I antibodies, and the activation levels of SHP2 and STAT1 were determined by western blot assay at indicated times post VSV infection. (b) Relative density of the blot in (a). (c) Relative VSV replication in macrophages in (a). (d) WT and MHC I^-/- mice were infected i.p. with VSV; viral load in the lung was calculated 18 h later. (e) BALF cell numbers in the lung from WT and MHC I^-/- mice infected with VSV. (f) H&E analysis of the VSV-infected lung from mice infected with VSV (200x). (g) Inflammation score of the lung damage in (f). Data are the of at least three independent experiments. Two-way ANOVA was adopted for statistical analysis in (b). Unpaired Student’s test was adopted for statistical analysis in (c), (d), and (e). and .

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