MTOR-Mediated Autophagy Is Involved in the Protective Effect of Ketamine on Allergic Airway Inflammation
Effect of rapamycin on autophagy in the lungs from asthmatic mice treated with 50 mg/kg ketamine. (a, b) Electron micrographs and quantification of autophagic vacuoles. (c–h) With Western blot, MTOR, p-MTOR, LC-3I, LC-3II, and Beclin-1 were determined and quantified. (i, j) With immunohistochemistry (magnification, ×200), p-MTOR were determined and quantified. Data are expressed as the mean ± SD of six mice per group. , versus the OVA groups; ,, and versus the OVA+Ket50 group.