Calcitriol Inhibits the Proliferation of Triple-Negative Breast Cancer Cells through a Mechanism Involving the Proinflammatory Cytokines IL-1β and TNF-α

Figure 2

Calcitriol, EB1089, IL-1β, and TNF-α effect on breast cancer cell proliferation. SUM-229PE (■), HCC1806 (●), and MCF7 (▲) cells were cultured in the absence (0) or presence of different concentrations of calcitriol (a), EB1089 (b), IL-1β (c), or TNF-α (d) during 6 days. Then, cell proliferation was evaluated by the XTT method. The results represent the average of 3 experiments, each in . vs. control. Nontreated cells were considered as 100% of cell proliferation. Ethanol was used as vehicle of calcitriol and EB1089 and phosphate-buffered saline solution (PBS) for cytokines.

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