Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

Figure 1

Sample preparation. (a) Tumor tissue was halved and treated with either Col D or Col NB8 together with DNase I using a gentleMACS Octo Dissociator and its predefined programs. After lysis of erythrocytes, the single-cell suspensions were stained with the designed panel of antibodies. The same gates were applied to cell populations originated from the tissues treated with Col D or Col NB8. (b) Samples of cells prepared from tumors were labeled with fluorescent anti-CD45 and anti-EpCAM antibodies before and after magnetic removal of epithelial cells. (c) Cells isolated from the tumor sample were stained with cisplatin for viability detection. The experiments were repeated with similar results.

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