Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

Figure 2

Stability of several antigens was affected by Pd-based barcoding. Ficoll-isolated PBMCs from a healthy donor were stained for live and dead cells and halved. One part of the cells was barcoded with the Cell-ID 20-Plex Pd Barcoding Kit, and the rest of the cells were used as a control without barcoding. Subsequently, the cells were stained with the designed panel of antibodies. The labeled cells were stored in the intercalator solution until measurement on CyTOF. After measurement, the barcoded cells were debarcoded according to the labeling pattern of the barcoding kit. (a) Markers with reduced intensity and (b) cell populations become more evident after Pd barcoding. The same gates (adjusted for the cells without barcoding) were applied to measured cells.

(a)

(b)