Implementation of Mass Cytometry for Immunoprofiling of Patients with Solid Tumors

Figure 4

Sample storage in PBS with 1% BSA. Tumor cells and PBMCs were isolated from a patient with HNSCC and stained for viability. After CD45-based barcoding, samples were halved. One part of the cells was stained with the panel of antibodies immediately, and the other part was stored in PBS with 1% BSA at 4°C overnight and labeled the next day. Dot plots display some representative markers of the blood sample. Gates were adjusted for cell samples stained immediately after isolation.