Iguratimod Inhibits the Aggressiveness of Rheumatoid Fibroblast-Like Synoviocytes

Figure 1

Iguratimod suppressed proliferation of RA-FLSs in a dose-dependent manner. RA-FLSs were starved for 24 h and then cultured in the presence of platelet-derived growth factor (PDGF) (10 ng/ml) with or without treatment with iguratimod at various concentrations for 72 h. Immunofluorescence staining for 5-ethynyl-2-deoxyuridine (Edu) (red, A), Hoechst 33342 (blue, B), and merged images (C) in the nucleus of cells were shown. Representative pictures (a) and the summarized data (b) of three separate experiments were shown. (c) The proliferation rate of RA-FLSs was also measured by the CCK-8 method. The data indicated the of 3 separate experiments (totally 6 RA-FLS lines). The data were analyzed using one-way ANOVA followed by Turkey’s test. versus the DMSO group. NS: not significant.

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