Journal of Immunology Research / 2019 / Article / Fig 7

Research Article

Immunoregulatory Effects of Subcutaneous Immunotherapy on Lymphocyte Subgroups and Cytokines in Children with Asthma

Figure 7

Changes in Th17 and Treg cell percentage and Th17/Treg ratio of children in both groups before and after treatment. (a) Flow cytometry was used to detect the percentage of Th17 cells by labeling peripheral blood with anti-CD4 and IL-17 (CD4+ IL-17+) fluorescent antibodies of subjects in the SCIT group at three time points; (b) Treg cells were similarly detected with anti-CD4, CD25, and CD127 (CD4+ CD25+ CD127-) antibodies; (c) changes in the Th17/Treg ratio were calculated from flow cytometry data for subjects in the SCIT and no-SCIT groups and plotted for the three time points. Designates a intergroup difference; designates a intergroup difference.

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