CD38 Deficiency Downregulates the Onset and Pathogenesis of Collagen-Induced Arthritis through the NF-κB Pathway

Figure 1

Alteration of the RelB gene expression and phenotype of BMDCs generated over 7 days from CD38^-/- mice. (a) Genotyping of CD38 gene deficiency in CD38^-/- mice (/group). DNA was extracted from tails of CD38^-/- mice as described in Materials and Methods. The CD38 and Neo gene expressions were determined by PCR. (b) Alteration of RelB gene expression in DCs from CD38^-/- mice (/group). DCs were cultured from the bone marrow of the WT and CD38^-/- mice as described in Materials and Methods. Gene expressions of CD38 and RelB were detected by RT-qPCR. (c) Phenotypes of DCs in CD38^-/- mice (/group). DCs were stained with antibodies against MHC II, CD40, and CD 80, respectively; the expression of above molecules was detected by flow cytometry. The data presented one of three independent experiments ().

(a)

(b)

(c)