The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice
Expression of costimulatory molecules on bone marrow-derived DCs from CBA. (a) Expression of H-2K on CD11c+ DCs. (b) Expression of CD86 on CD11c+H-2k+ DCs. (c) Expression of CD80 on CD11c+H-2k+ DCs. (d) Expression of CD80/86 on CD11c+H-2k+ DCs. mice per group. At least three independent experiments were performed. The arrows show significant intergroup differences (). Notes: GM-CSF+IL-4—BMCs cultured in the presence of GM-CSF and IL-4 alone; Bay—BMCs cultured in the presence of GM-CSF, IL-4, and BAY11-7082 added on day 2 of culturing; IL-10—BMCs cultured in the presence of GM-CSF, IL-4, and IL-10 added on day 3 of culturing; TGF-β—BMCs cultured in the presence of GM-CSF, IL-4, and TGF-β added on day 3 of culturing.