The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice

Figure 4

Expression of Treg markers in the cocultures of splenocytes and DCs from C57BL/6 and CBA mice. (a) The relative frequency of CD4+CD25+ cells in C57Bl/6 mice. (b) The relative frequency of FoxP3+ cells within CD4+CD25+ cells in C57Bl/6 mice. (c) The relative frequency of CD4+CD25+ cells in CBA mice. (d) The relative frequency of FoxP3+ cells in the CD4+CD25+ population in CBA mice. mice per group. At least three independent experiments were performed. The arrows show significant intergroup differences (). Note: DC+Spl—coculture of immature DCs and splenocytes; DC-Bay+Spl—coculture of DCs treated with BAY 11-7082 and splenocytes; DC-IL-10+Spl—coculture of DCs treated with IL-10 and splenocytes; DC-TGF-β+Spl—coculture of DCs treated TGF-β and splenocytes; DC (Bay+IL-10)+Spl—coculture of DCs treated with BAY 11-7082 and IL-10 and splenocytes; DC (Bay+TGFβ)+Spl—cocultures of DCs treated with a combination of BAY 11-7082 and TGF-β and splenocytes.

(a)

(b)

(c)

(d)