The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice
The frequency of IL-10-producing cells among CD4+ lymphocytes in the coculture of splenocytes and DCs in (a) C57Bl/6 mice and (b) CBA mice. mice per group. At least three independent experiments were performed. The arrows show significant intergroup differences (, ). Note: DC+Spl—coculture of immature DCs and splenocytes; DC-Bay+Spl—coculture of DCs treated with BAY 11-7082 and splenocytes; DC-IL-10+Spl—coculture of DCs treated with IL-10 and splenocytes; DC-TGF-β+Spl—coculture of DCs treated with TGF-β and splenocytes; DC (Bay+IL-10)+Spl—coculture of DCs treated with BAY 11-7082 and IL-10 and splenocytes; DC (Bay+TGFβ)+Spl—coculture of DCs treated with BAY 11-7082 and TGF-β and splenocytes.