Induction of Macrophage M2b/c Polarization by Adipose Tissue-Derived Mesenchymal Stem Cells

Figure 5

IL-12 p40 expression in different groups of Mφ. (a) WB and its gray relative analysis. Nonactivated Mφ upregulate the expression of IL-12 p40 after LPS induction. Both the nonactivated Mφ and the LPS-induced M1-Mφ downregulate the expression of IL-12 p40 after ADMSC-CM induction. The expression of IL-12 p40 in the IL and IL-CM groups was very low. (b) The relative fluorescence intensity analysis. The expression of IL-12 was effectively reduced after ADMSC induction. The change of fluorescence intensity of other groups was not obvious.