Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

Figure 5

Effects of hemocyanins in combination with QS-21 on the viability of the MOC7 and AT-84 E7 Luc oral cancer cell lines. Cells ( cells/well) were incubated with different concentrations of hemocyanin (KLH or FLH) and QS-21 for 24 and 72 hours, and viability was determined using the alamarBlue® method. (a) Effects on MOC7 cell viability with KLH or FLH after 24 hours of culture. (b) Effects on MOC7 cell viability with KLH or FLH after 72 hours of culture. (c) Effects on MOC7 cell viability with KLH or FLH in combination with QS-21 after 24 hours of culture. The concentration gradient of this adjuvant, described in Methods, is indicated. (d) Effects on MOC7 cell viability with KLH or FLH in combination with QS-21 after 72 hours of culture. (e) Effects on AT-87 Luc cell viability with KLH or FLH after 24 hours of culture. (f) Effects on AT-87 Luc cell viability with KLH or FLH after 72 hours of culture. (g) Effects on AT-87 Luc cell viability with KLH or FLH in combination with QS-21 after 24 hours of culture. The concentration gradient of this adjuvant, described in Methods, is indicated. (h) Effects on MOC7 cell viability with KLH or FLH in combination with QS-21 after 72 hours of culture. In each case, the cell viability values obtained at different formulation concentrations were compared with those obtained for the corresponding untreated condition. , one-way ANOVA; independent experiments performed in triplicate.