Lipid-Rich Extract from Mexican Avocado Seed (Persea americana var. drymifolia) Reduces Staphylococcus aureus Internalization and Regulates Innate Immune Response in Bovine Mammary Epithelial Cells

Table 2

Bovine oligonucleotides used in this study.

Specificity	Primer	Sequence (5-3)	Fragment size (bp)	Annealing temperature (°C)	References
IL-1β	Forward	GCAGAAGGGAAGGGAAGAATGTAG	198	52	[19]
	Reverse	CAGGCTGGCTTTGAGTGAGTAGAA
IL-6	Forward	AACCACTCCAGCCACAAACACT	179	57	[19]
	Reverse	GAATGCCCAGGAACTACCACAA
TNF-α	Forward	CCCCTGGAGATAACCTCCCA	101	56	[19]
	Reverse	CAGACGGGAGACAGGAGAGC
IL-10	Forward	GATGCGAGCACCCTGTCTGA	129	59	[19]
	Reverse	GCTGTGCAGTTGGTCCTTCATT
BNBD5	Forward	GCCAGCTGAGGCTCCATC	143	55	[9]
	Reverse	TTGCCAGGGCACGAGATCG
DEFB1	Forward	CCATCACCTGCTCCTCACA	185	54	[9]
	Reverse	ACCTCCACCTGCAGCATT
TAP	Forward	GCGCTCCTCTTCCTGGTCCTG	216	57	[9]
	Reverse	GCACGTTCTGACTGGGCATTGA
GAPDH	Forward	TCAACGGGAAGCTCACTGG	237	56.9	[9]
	Reverse	CCCCAGCATCGAAGGTAGA