Research Article

Cross-Reaction, Enhancement, and Neutralization Activity of Dengue Virus Antibodies against Zika Virus: A Study in the Mexican Population

Figure 2

Expression and purification of rE-ZIKV in S2 cells. (a) Cytoplasmic expression of rE-ZIKV in Drosophila melanogaster S2 cells after transfection with pMT/E-ZIKV and induction with CuSO4. The envelope protein was detected using a monoclonal antibody anti-E protein of DENV (C-21), which cross-reacts with E-ZIKV. As a positive control, S2 cells were transfected with a plasmid encoding the green fluorescent protein. (b) Secretion kinetics of the rE-ZIKV protein from day 6 to 16 after induction with CuSO4. The supernatants of the stably transfected S2 cells were harvested and analyzed using western blot analysis. The immunodetection was performed with anti-E protein (C-21).