Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice

Figure 6

Budesonide pretreatment suppressed NLRP3 inflammasome activation in the lungs of LPS-treated mice. Twelve hours after LPS administration, the mRNA expression levels of NLRP3 (a), pro-caspase-1 (b), pro-IL-1β (f), and pro-IL-18 (g) were detected using real-time PCR. The protein expression of levels NLRP3 (c, d) and caspase-1 p20 (c, e) were detected using western blotting. The concentrations of IL-1β (h) and IL-18 (i) in the BALF were detected using ELISA. Data were expressed as deviations (). compared with the control group, compared with the ALI group, and compared with the ALI group.

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