Overexpression of Tumor Necrosis Factor-Like Ligand 1 A in Myeloid Cells Aggravates Liver Fibrosis in Mice
TL1A was upregulated in liver tissues and macrophages in mice with hepatic fibrosis. (a) The hepatic fibrosis model was established and verified by H&E and Sirius Red staining (200x). (b) The relative quantification of TL1A mRNA was measured by RT-PCR. (c, d) TL1A expression in the CCl4/WT group was significantly higher than those in the Control/WT and Oil/WT group, which was markedly higher in the CCl4/Tg group than that in the CCl4/WT group detected by Western blot. (e, f) TL1A was discovered in macrophage through F4/80 and TL1A immunofluorescence double staining, which was markedly higher in the CCl4/WT group than in the Oil/WT group and significantly higher in the CCl4/Tg group than in the CCl4/WT group. Data are expressed as mean ± SD, and .