Research Article
Overexpression of Tumor Necrosis Factor-Like Ligand 1 A in Myeloid Cells Aggravates Liver Fibrosis in Mice
Figure 6
Constitutive TL1A expression on macrophages upregulated the PDGF-BB, TNF-α, and IL-1β in the serum, liver tissues, and macrophages. (a) The expression of TGF-β1 in serum, liver tissue, and the medium of BMMs and PMs was detected by ELISA and RT-PCR. Serum TGF-β1 in the CCl4/Tg group were significantly higher than those in the CCl4/WT group, and TGF-β1 mRNA in the CCl4/Tg group were also higher than those in the CCl4/WT group. But there was no statistical significance between the Tg group and WT group of CM derived from BMMs and PMs. (b, c, d) The expressions of PDGF-BB, TNF-α, and IL-1β in serum, liver tissue, and the medium of BMMs and PMs were detected by ELISA and RT-PCR, respectively. The cytokines in serum and mRNA in liver tissue in the CCl4/Tg group were significantly higher than those in the CCl4/WT group. And those derived from BMM and PM supernatant in the LPS + IFN-γ + M-CSF/Tg group were significantly higher than those in the LPS + IFN-γ + M-CSF/WT group. Data are expressed as mean ± SD, and .
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