Human Hepatocytes and Differentiated Adult-Derived Human Liver Stem/Progenitor Cells Display In Vitro Immunosuppressive Properties Mediated, at Least in Part, through the Nonclassical HLA Class I Molecule HLA-G

Figure 7

The in vitro immune inhibitory properties of liver cells treated with OSM. ADHLSCs previously treated with OSM or IFN-γ were added as third-party cells to MLR cultures at the ratio of 1 : 0.5 for 7 days. Differentiated ADHLSCs were used as a control. The proliferation of allogeneic responder PBMCs was evaluated by the [³H]-thymidine incorporation method. The proliferation obtained in the MLR was arbitrarily assigned a value of 100% and used as a reference to calculate the percentage of proliferation obtained with the other conditions. The bars represent the of at least 3 independent experiments. The data were analyzed using the one-way ANOVA test followed by the Tukey post hoc test for multiple comparisons of the MLR group to the other treatment groups. and .