Research Article
Immunoregulatory Effect of Koumine on Nonalcoholic Fatty Liver Disease Rats
Figure 4
Koumine reduces the production and mRNA expression of cytokines in activated T cells. (a) Model and 1.4 mg/kg koumine-treated rats were sacrificed to obtain the serum and measured for the cytokines by multifactor detection of flow cytometry. (b) The livers were isolated from model and koumine-treated rats, and after being homogenized, the supernatants were harvested to measure the cytokine production by ELISA. (c) The livers were isolated from model and koumine-treated rats, and mRNA expression of selected genes were measured by real-time PCR. Data are (). and versus model. Data presented are representative of three independent experiments.
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