Promiscuous Chemokine Antagonist (BKT130) Suppresses Laser-Induced Choroidal Neovascularization by Inhibition of Monocyte Recruitment

Figure 2

In vivo assessment of BKT130’s effect in a rodent model of LI-CNV. BKT130 was injected intravitreally in a rat model of LI-CNV ( eyes). The eyes injected with PBS served as a negative control (), while intravitreal injections of aflibercept served as the positive control (). BKT130 was also injected with aflibercept to assess an additive effect (). CNV was identified and quantified using a fluorescent microscope in isolectin-stained RPE-choroid flat mounts (a–d). Each laser treated area was observed in a 20x lens and the whole flat mount in 4x lens. The CNV area was measured and compared between treatments and between PBS-injected eyes (e). The -axis presents the averaged (±SEM) CNV area (mm²) of treated and PBS-injected control eyes. and .