Promiscuous Chemokine Antagonist (BKT130) Suppresses Laser-Induced Choroidal Neovascularization by Inhibition of Monocyte Recruitment

<div>Gene expression profile of the retinas and RPE-choroid following treatment. mRNA expression levels of genes related to angiogenesis (<i>VEGFA</i>, <i>IL1β</i>, and <i>TNFα</i>), inflammation (<i>CCL2</i>, <i>CCR2</i>, <i>CCL5</i>, <i>TNFα</i>, <i>NAP-2</i>, and <i>MIP-2</i>), mononuclear cell markers (<i>NOS1</i>, <i>CD163</i>, and <i>CD11b</i>), and macrophage recruitment (<i>CCL2</i>, <i>CCR2</i>, <i>NAP-2</i>, and <i>MIP-2</i>) were evaluated in the RPE-choroid (a–d) and in the retinas (e–h) of rats via QPCR (<svg height="8.55521pt" id="M55" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.158,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.421,0)"><path d="M244 635C114 635 38 519 38 422C38 317 111 240 217 240C236 240 255 244 277 256L345 292C311 140 203 39 59 15L64 -15C89 -15 150 -5 204 17C339 72 440 202 440 386C440 521 368 635 244 635ZM228 602C326 602 352 479 352 390C352 370 351 347 348 324C327 308 293 296 258 296C174 296 124 369 124 458C124 517 152 602 228 602Z"></path></g></svg> eyes in each group: PBS, BKT130, aflibercept, and BKT130+aflibercept). Presented are the genes that significantly changed after treatment. 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Journal of Immunology Research

fig3

Figure 3

Figure 3: Promiscuous Chemokine Antagonist (BKT130) Suppresses Laser-Induced Choroidal Neovascularization by Inhibition of Monocyte Recruitment 