High Thymic Output of Effector CD4+ Cells May Lead to a Treg : T Effector Imbalance in the Periphery in NOD Mice
NOD and B6 CD4+ Tregs exhibit equivalent suppressive activity in vitro. (a) Percentages of CD4+CD25+ cells that express Foxp3+ in peripheral lymph nodes of female NOD and B6 mice at varying ages (-10). (b) Sample histograms of Foxp3 expression in CD4+CD25+ cells from peripheral lymph nodes of 12-week-old female B6 and NOD mice. (c) Lymphoid cells from NOD and B6 mice were collected at 12 weeks of age and CD4+CD25+ cells sorted. Varying numbers of CD4+CD25+ cells were cocultured with age-matched NOD CD4+CD25- responder cells, irradiated splenocytes, and anti-CD3 in vitro. Proliferation was determined by detection of H3-thymidine uptake. denotes a significant difference from positive control at .
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