Figure 2: Phenotypic characterization of circulating Th1, Treg, and DCs and expression levels of PD-1 and PD-L1 on immune cells in CHB patients. This panel is based on surface markers CD3, CD8, IFN-γ, CD4, CD25, Foxp3, LIN1, HLA-DR, PD-1, and PD-L1. All cells were gated using a FSC-H/SSC-A dot plot. (a) Among PBMCs, CD3+CD8 cells were defined as CD4+ T cells. Among CD4+ T cells, CD4+IFN-γ+ cells were Th1 and then MFI of PD-1 expression on Th1 was analyzed. (b) Among PBMCs, CD4+ cells were gated and then CD4+CD25+FoxP3+ cells were defined as Treg. MFI of PD-1 expression on Treg was analyzed. (c) Among PBMCs, LIN1 cells were gated and then LIN1HLA-DR+ cells were defined as DCs. MFI of PD-L1 expression on DCs was analyzed.