Skin-Resident γδ T Cells Exhibit Site-Specific Morphology and Activation States

Figure 2

Fluorescent immunohistochemical analysis of skin-resident γδ T cells in cryosectioned tissue. Frozen cryosections of skin obtained from the murine ear, back, and footpad were stained using anti-TCRγ/δ antibody and visualized using cryosection immunofluorescence. As in whole-mount sections, γδ T cells in the skin show variable morphological characteristics dependent on skin site. TCRγ/δ-immunopositive cells (indicated by white arrows) are found primarily in the epidermis of the ear (a), back (b), and footpad (c). There is an increased background staining in the epidermis of the footpad (indicated by black arrow), though cells are not found in this area. Insets are enlarged representations of γδ T cells from the three skin sites. Scale bar = 50 μm.

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