Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens
Expression of recombinant CL-12 in the Flp-In™-CHO cells. Supernatants were harvested from the cultivation of the Flp-In™-CHO cells expressing rCL-12ED. Culture supernatants were then subjected to 4-12% SDS-PAGE and analyzed with CL-12 pAb (a) or anti-His mAb (b) by western blot. Lane 1, rCL-12R&D; lane 2, culture supernatant.