Human Papillomavirus Type 16 Disables the Increased Natural Killer Cells in Early Lesions of the Cervix

Figure 3

The cytotoxicity of NK cells derived from HPV16⁺ or HPV18⁺ women using HeLa cells labeled with CFSE. CD56⁺CD16⁺ NK cells were isolated from cervical brush specimens of women infected with either HPV16 or HPV18, respectively, via FACS. NK cells were cocultured with CFSE-HeLa in designated ratios in 24-well plates and incubated at a 37°C incubator for 4-6 hours. Cells were harvested and stained with 7-AAD. CFSE⁺7-AAD⁺ cells were examined by FACS. ; .