Research Article

Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro

Figure 1

OECCL express OEC-associated antigens and increase DAMP production under heat shock treatment. (a) Representative histograms for CA-125, MUC1, survivin, ErbB-2, and CEA expression in four OECCL (CAOV3, SKOV-3, Hey, and A2780) evaluated by flow cytometry. The upper histograms indicate isotype control staining. (b) The levels of the plasma membrane translocated calreticulin (surface CRT, left panel) and the HMGB1 in the supernatant (right panel) of heat shock-treated (white bars) or control (black bars) OEC cells. The results were obtained from multiple independent experiments. , , and #.
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