Research Article
Effect of Ionizing Radiation on Human EA.hy926 Endothelial Cells under Inflammatory Conditions and Their Interactions with A549 Tumour Cells
Figure 4
Analysis of tumour cell migration by in vitro scratch assay. (a) Quantification of the wounded area. The results show what percentage of the originally free area (100%) was not covered by cells. Relative cell adhesion is presented as (SD) from four independent experiments, obtained in duplicates for each approach; asterisks illustrate significance: , , and . (b) Representative phase contrast photographs of the wounded area taken immediately after the scratch (0 hours) as well as 24 hours and 48 hours after IR, without or with TNF-α treatment of the cells. Black arrows point out the altered cell morphology observed in cells 48 hours after irradiation with 0.5 Gy and stimulation with TNF-α.
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