Effect of TNF-α-Induced Sclerostin on Osteocytes during Orthodontic Tooth Movement

Figure 3

TNF-α enhanced osteoclastogenesis and sclerostin expression in osteocytes in vivo. (a) Images: histological sections of calvariae were obtained from wild-type mice after 5 days of daily supracalvarial administration of phosphate-buffered saline (PBS) or 3.0 μg/day tumor necrosis factor alpha (TNF-α). Sections were stained with tartrate-resistant acid phosphatase (TRAP); cells stained red are regarded as TRAP-positive. . Graph: the numbers of TRAP-positive cells on the calvariae (; ). (b) Images: sections were immunolocalized with antibodies specific for sclerostin, then counterstained with hematoxylin. . Graph: the numbers of sclerostin-positive osteocytes on the calvariae (; ). (c) SOST mRNA levels in mouse calvariae detected using real-time reverse transcription polymerase chain reaction.

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