Reverse Immunology Approach to Define a New HIV-gp41-Neutralizing Epitope

Figure 1

Reverse immunology applied to an epitope-based vaccination strategy. Mice immunized with the W614A-3S peptide were used to generate mAbs. Clones that reacted with the vaccine peptide were studied for their HIV-1-neutralizing properties. The neutralizing F8 mAb was then used to screen a phage peptide library for mimotopes of the F8 epitope. Finally, vaccination of mice with the selected mimotope induced neutralizing Abs in the serum.