Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage

Figure 1

CpG ODN induced macrophage activation. (a) Representative phase-contrast images of RAW264.7 cells stimulated with CpG ODN1826 (1 μM) for 0 h, 4 h, 8 h, and 16 h. (b, c) The RAW264.7 cells were treated with CpG ODN1826 (1 μM) for 16 h. And then, the mRNA and protein expression levels of TNF-α, IL-1β, and IL-8 were determined by qRT-PCR (b) and ELISA (c). (d) Inflammation-related gene expression levels were altered in the process of CpG ODN1826-induced macrophage activation from 0 h to 16 h. (e) Nitrite was determined in the supernatant of RAW264.7 cells stimulated with CpG ODN1826 for 16 h. (f) iNOS protein levels were detected by Western blot in RAW264.7 cells after stimulation with CpG ODN1826 for 16 h. .

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