Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4⁺ T Helper Cell Responses

Figure 1

Adenosine treatment induced NLRP11 expression at both the mRNA and protein levels in B lymphoblasts. (a) NLRP11 mRNA expression after 4 and 10 hours of stimulation with PMA/ionomycin (50 ng/500 ng/ml), CD40L (1 μ/ml), LPS (100 ng/ml), and adenosine (50 μM) ( indicates significance at ; stimulated vs. nonstimulated). (b) NLRP11 protein expression after 4 and 10 hours of stimulation with PMA/ionomycin (50 ng/500 ng/ml), CD40L (1 μ/ml), LPS (100 ng/ml), and adenosine (50 μM). (c) NLRP11 protein expression levels at 0.0, 25, 50, 100, and 200 μM adenosine. (d) NLRP11 protein expression levels at 1, 2, 3, 4, 5, 6, 7, and 8 hours post stimulation with 50 μM adenosine. Results are representative of four independent experiments. Student’s -test shows the significant difference between stimulated and nonstimulated cells. indicates , , and .

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