Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4+ T Helper Cell Responses
Coculturing with NRLP11 siRNA-transfected Daudi cells restored Th1 and Th17 cell responses but did not alter Th2 and Treg responses. (a) Costimulatory molecules CD80 and CD86 surface expressions were determined by a flow cytometer. (b) NLRP11 mRNA expression in Daudi cells that were transfected with scrambled siRNA or cotransfected with the mixture of S1, S2, and S3 siRNAs. Data are presented as percentages compared with the negative siRNA. (c) 24 hours after transfection, Daudi cells were treated with adenosine and cell and supernatants were harvested following 4 hours of stimulation. IFN-γ, IL-17A, IL-10, and IL-13 levels were measured in the supernatants by ELISA. (d) T-bet, GATA3, RORγt, and FOXP3 gene expressions were measured by QPCR. Data are presented as fold changes compared with the control. Experiments were carried out in duplicates. Results are representative of three independent experiments. Fold changes were depicted on the axis, which shows the significant difference between negative siRNA transfected and siRNA transfected. Values represent the and are representative of four separate experiments. Student’s -test shows the significant difference between stimulated and nonstimulated cells. indicates ,, and .
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