Journal of Immunology Research / 2020 / Article / Fig 5

Research Article

Adenosine-Induced NLRP11 in B Lymphoblasts Suppresses Human CD4+ T Helper Cell Responses

Figure 5

Adenosine induces NLRP11 and A2B receptor gene expression and elevates endogenous ERK1/2 and phosphorylated Akt protein levels in Burkitt’s lymphoma cells. (a) A1, A2A, A2B, and A3AR fold changes in adenosine-induced human Daudi cells at 4 hours post stimulation. (b and c) After adenosine and caffeine treatment, cell lysates were harvested and total protein was separated by SDS-PAGE. Blots were probed with anti-NLRP11, ERK1/2, Akt, pAkt (Thr308), and pAkt (Ser473) antibodies and visualized using ECL after incubation with an anti-rat HRP-conjugated antibody. Images are representative of two independent experiments.

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