Reaction of Lectin-Specific Antibody with Human Tissue: Possible Contributions to Autoimmunity

Table 1

Reaction of unimmunized goat serum and affinity-purified polyclonal goat anti-lectin antibodies with different tissue antigens expressed as ELISA index.

Antigen	Controls	WGA	PNA	SBA	PHA
Parietal cell	0.41 -	1.00 ++	0.42 -	0.41 -	0.29 -
Intrinsic factor	0.26 -	1.10 ++	0.37 -	0.40 -	2.00 +++
ASCA+ANCA	0.34 -	1.80 +++	0.43 +	0.55 +	0.40 -
Calprotectin	0.17 -	1.00 ++	0.46 +	0.29 -	1.80 +++
DPP-IV	0.38 -	1.10 ++	0.22 -	0.41 -	0.81 ++
21-Hydroxylase	0.22 -	0.85 ++	0.41 -	0.33 -	0.22 -
Hepatocytes	0.33 -	1.30 ++	0.39 -	0.56 +	2.30 ++++
Somatotropin	0.48 +	1.90 +++	0.50 +	0.60 +	0.90 ++
Enteric nerve	0.21 -	0.42 -	0.72 +	0.50 +	0.22 -
Zonulin	0.39 -	2.70 ++++	0.37 -	0.60 +	0.31 -
MBP	0.40 -	1.75 +++	0.60 +	0.64 +	0.71 +
α+β Tubulin	0.17 -	0.50 +	0.32 -	0.21 -	0.72 +
Neurofilaments	0.35 -	1.10 ++	0.18 -	0.37 -	1.42 +++
Asialoganglioside	0.31 -	0.95 ++	0.48 +	0.36 -	2.90 ++++
Synapsin	0.24 -	0.80 +	0.60 +	0.42 -	0.32 -
Human aquaporin	0.33 -	0.90 ++	0.51 +	0.38 -	0.30 -
Amyloid-β	0.15 -	1.10 ++	0.35 -	0.44 +	0.20 -
Neurotrophin	0.23 -	1.70 +++	0.26 -	0.60 +	0.85 ++
Neurocrescin	0.18 -	2.20 ++++	0.25 -	0.46 +	0.20 -
Rabaptin-5	0.24 -	0.84 ++	0.50 +	0.45 +	0.20 -
Presenilin	0.11 -	1.50 +++	0.80 +	0.67 +	0.53 +
Tau protein	0.16 -	1.80 +++	0.20 -	0.32 -	0.60 +
BDNF	0.18 -	0.81 ++	0.21 -	0.48 +	1.30 ++
Beta-NGF	0.25 -	1.10 ++	0.22 -	0.31 -	0.80 +
GAD-65	0.10 -	0.50 +	0.44 +	0.37 -	0.19 -
Tyrosinase	0.26 -	1.20 ++	0.28 -	1.60 +++	1.52 +++
TPO	0.39 -	3.40 +++++	0.44 +	0.90 ++	3.60 +++++
Enolase	0.41 -	2.00 +++	0.33 -	0.47 +	0.71 +
Elastin	0.20 -	0.96 ++	0.22 -	0.41 -	0.38 -
Calmodulin	0.29 -	2.45 ++++	0.31 -	0.53 +	1.34 +++
Platelet glycoprotein	0.36 -	1.30 ++	0.38 -	0.63 +	1.10 ++
Fibulin	0.34 -	1.10 ++	0.55 +	0.35 -	1.00 ++
Ovary	0.21 -	2.36 ++++	0.78 +	0.65 +	0.42 -
Insulin+islet cell	0.19 -	2.31 ++++	0.80 +	0.70 +	0.41 -
α-Myosin	0.37 -	2.00 +++	0.45 +	0.88 ++	1.62 +++
Acetylcholine-R	0.32 -	1.43 +++	0.50 +	0.41 -	0.42 -
Dopamine-R	0.17 -	1.90 +++	1.00 ++	0.58 +	1.10 ++
Glutamate-R	0.21 -	0.99 ++	1.10 ++	0.37 -	0.50 +
Other tissues	0.26 ± 0.18	0.21 ± 0.13	0.19 ± 0.15	0.22 ± 0.16	0.28 ± 0.17
-	+	++	+++	++++	+++++
0.00-0.42	0.43-0.80	0.81-1.3	1.31-2.0	2.1-3.0	>3
Negative	Borderline	Low	Moderate	Strong	Very strong