Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response
Formation of perivascular infiltrates of CD4/CD8+ T clusters in inflammation-experienced gastric subserous tissue. Six to eight-week-old female C57BL/6 mice were vaccinated in the gastric subserous layer. (a) 28 days later, the gastric tissue was exposed and a white lump was observed. (b, c) Frozen sections of gastric tissue from immunized or naïve mice were stained with antibodies against CD31 (red); nuclei are depicted by DAPI stained blue. Scale bars: 100 μm. The fluorescence images were acquired with a Panoramic 250 Flash III Scanner (3DHistech); counts of CD31 per 0.36 mm2 were randomly chosen and calculated. Values are the ().. (d) Frozen sections of gastric tissue from immunized mice were stained with antibodies against CD31 (red), CD90.2, CD4, CD8, CD11b, or MHCII (green); nuclei were depicted by DAPI stained blue. Scale bars: 200 μm.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.