Journal of Immunology Research / 2020 / Article / Fig 5

Research Article

Perivascular Lymphocyte Clusters Induced by Gastric Subserous Layer Vaccination Mediate Optimal Immunity against Helicobacter through Facilitating Immune Cell Infiltration and Local Antibody Response

Figure 5

Perivascular lymphocyte clusters enhanced antigen-specific sIgA antibody response. All mice were performed with laparotomy to access the stomach and injected with alum-based vaccine into GSL. At day 28, these immunized mice were divided into two groups. For one group, half of the mice were administered with anti-CD90.2 antibody and cohoused with the other half. At day 42, the anti-CD90.2 antibody-treated mice and the other mice not injected with an antibody were surgically jointed. At day 56, the indicated partner was infected with H. felis. Another group was treated the same way but used IgG2b isotype. (a, b) After the later challenge on day 3, those mice were harvested and analyzed. The antigen-specific antibodies from the blood or gastric tissue were measured using ELISA. (c–e) After the later challenge at day 7, those mice were harvested and analyzed. The antigen-specific IgM, IgG, and IgA from gastric tissue were measured using ELISA. The absorbance value was detected at 450 nm using a plate reader. Values were the (). All indicated values were tested using the ANOVA analyses or t tests. , , and .

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