Epigenetic Therapy as a Putative Molecular Target to Modulate B Cell Biology and Behavior in the Context of Immunological Disorders

Figure 3

HDAC activity is necessary for peritoneal homeostasis and B cell function. C57BL/6 male mice were injected with iHDAC (TSA 100 nM) in the peritoneal cavity, and upon 5 days, peritoneal cells (a–c) and the mesenteric lamina (d–f) were analyzed. B1 cells were specifically increased in iHDAC-treated groups (c). The mesenteric lamina structure was disrupted, and degranulated mast cells were observed ((e, E), red arrow). ELISA assay showed that while iHDAC-treated B cells secreted higher levels of IgM, the levels of IgA did not change under the same conditions. Degranulated mast cells were observed only in the presence of iHDAC (B, E). Data are represented as and are representative of 4 animals in each group. Proliferative cluster (red arrow (b)). .