Prognostic and Clinical Value of Interleukin 6 and CD45⁺CD14⁺ Inflammatory Cells with PD-L1⁺/PD-L2⁺ Expression in Patients with Different Manifestation of Ovarian Cancer

Figure 1

Flow cytometry analysis of CD45⁺CD14⁺ cells expressing PD-L1 or PD-L2. An acquisition gate was established based on FSC and SSC that included mononuclear cells. A population P1 was drawn around MNCs (a). Next, the P1 gated events were analyzed for CD45 FITC and CD14 Pe-Cy7 staining and positive cells (CD45⁺CD14⁺) were gated (region Q2) (b). The final dot plots CD45⁺CD14⁺ expressing (d) PD-L1 or (f) PD-L2 versus FMO control were established by combined gating of events using population P1 and region Q2-1 or Q2-2. The number in the upper right quadrant on the dot plots (d, e) represents the percentage of CD45⁺CD14⁺ expressing (d) PD-L1 or (e) PD-L2.