M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin

Figure 5

(a) E-cadherin and cofilin expression in AGS. AGS cells were plated in 6-well plates containing cover glass for 24 h. After 24 h of incubation, AGS cells were treated with 10 μg/ml of 5FU and the inserts containing polarized THP-1-M2 macrophages were added to the AGS wells. AGS cells were then stained with DAPI and with E-cadherin, β-actin, and cofilin antibodies. The stained cells were observed under a confocal microscope. (b) Western blot analysis of EMT factors. Western blot analysis of E-cadherin, vimentin, ZEB-1, Snail, integrin β3, pFAK, and cofilin expression. (c) Quantification of western blot bands. Statistically significant difference was set at , .

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