Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway

Figure 6

Gene silencing in LBP induced DCs using Blimp1-specific siRNA. DCs were transfected with siRNA-Prdm1-1, siRNA-Prdm1-2, siRNA-Prdm1-3, siRNA-Prdm1-4, siRNA-NC, reagent alone (mock-treated), or nontransfected cells (control). After coculture of transfected DCs with LBP for 24 h, the expression of Blimp1 was detected by Western blot (a and b), and the IL-6 concentration in the culture supernatant was detected by ELISA (c). β-Actin expression was treated as internal reference. Values are the mean and SD of 3 independent experiments. , , and versus control.

(a)

(b)

(c)