The Effectiveness of Rapamycin Combined with Eltrombopag in Murine Models of Immune-Mediated Bone Marrow Failure

Figure 7

Efficacy of rapamycin combined with eltrombopag on regulatory T cells. (a) The ratio of regulatory T cells increased notably in the AA group after receiving CsA, Rapa, or Rapa+Elt, respectively, and there was a significant difference between the Rapa group and AA group, as well as the Rapa+Elt group and AA group (). There was no significant difference between the effect on numbers of Tregs of the Rapa and Rapa+Elt groups. No significant upregulation of the CD152 expression was observed on Tregs in the AA group after receiving Rapa or Rapa+Elt, respectively. As for IL-10, Rapa can increase the secretion of IL-10 from Treg cells compared with the CsA group and had a better outcome when combined with Elt in AA mice. (b) Representative flow cytometry analyses of the secretion IL-10 in Tregs and Tregs/PBMNC ratio after Rapa alone and combined with Elt treatment.

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