CD40 Signaling Promotes CXCR5 Expression in B Cells via Noncanonical NF-κB Pathway Activation

Figure 1

CXCR5 expression was reduced in B cells in vitro culture. Splenocytes were in vitro cultured for 24, 48, 72, and 96 hours. (a) Cells were stained with CD19-FITC and CXCR5-APC; (b) Representative flow cytometry data plots showed the levels of CXCR5 on CD19⁺ cells or CD19^- cells (representing three independent experiments, ); (c) Representative flow cytometry data plots showed the mean fluorescence intensity (MFI) of CXCR5 on CD19⁺ cells in vitro cultured for 24, 48, 72, and 96 hours (representing three independent experiments, ); (d) Flow cytometry data statistics showed the frequencies of CXCR5⁺ B cells (representing three independent experiments, ). Living cells were gated according to forward scatter (FSC) and side scatter (SSC) parameters. All flow cytometry results were analysed and plotted using Fluorescence Minus One controls (FMO). , NS indicating not significant (ANOVA test).

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