Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring

Figure 2

Gating strategy. Representative dot plots of cPBMC (a) and WB (b) samples: after selecting the window of instrumental stable acquisition time lapse and singlet events, lymphocytes were gated within live cells (a) or CD45+ cells/leukocytes (b). CD3+ cells were subsequently identified within the lymphocyte gate (common gate for (a) and (b) analyses) and further distinguished in CD4+ and CD8+ lymphocytes. After that, naïve/memory subsets were identified within CD3+, CD4+, and CD8+ T cells through CD45RA/CCR7 combination (c).